Brabham, Robin Louis, Spears, Richard James, Walton, Julia et al. (3 more authors) (2018) Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification. Chemical Communications. pp. 1501-1504. ISSN 1364-548X
Abstract
Protein bioconjugation frequently makes use of aldehydes as reactive handles, with methods for their installation being highly valued. Here a new, powerful strategy to unmask a reactive protein aldehyde is presented. A genetically encoded caged glyoxyl aldehyde, situated in solvent-accessible locations, can be rapidly decade through treatment with just one equivalent of allylpalladium(II) chloride dimer at physiological pH. The protein aldehyde can undergo subsequent oxime ligation for site-selective protein modification. Quick yet mild conditions, orthogonality and powerful exposed reactivity make this strategy of great potential in protein modification.
Metadata
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Institution: | The University of York | ||||||
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) | ||||||
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Depositing User: | Pure (York) | ||||||
Date Deposited: | 22 Mar 2018 09:30 | ||||||
Last Modified: | 06 Jan 2024 00:32 | ||||||
Status: | Published | ||||||
Refereed: | Yes | ||||||
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Filename: C7CC07740H.pdf
Description: Palladium-unleashed proteins: gentle aldehyde decaging for site-selective protein modification
Licence: CC-BY 2.5