Melling, G.E., Flannery, S.E., Abidin, S.A. et al. (13 more authors) (2018) A miRNA-145/TGF-β1 Negative Feedback Loop Regulates the Cancer-Associated Fibroblast Phenotype. Carcinogenesis, 39 (6). pp. 798-807. ISSN 0143-3334
Abstract
The dissemination of cancer cells to local and distant sites depends on a complex and poorly understood interplay between malignant cells and the cellular and non-cellular components surrounding them, collectively termed the tumour microenvironment. One of the most abundant cell types of the tumour microenvironment is the fibroblast, which becomes corrupted by locally derived cues such as TGF-β1 and acquires an altered, heterogeneous phenotype (cancer-associated fibroblast, CAF) supportive of tumour cell invasion and metastasis. Efforts to develop new treatments targeting the tumour mesenchyme are hampered by a poor understanding of the mechanisms underlying the development of CAF.Here we examine the contribution of microRNA to the development of experimentally-derived CAF and correlate this with changes observed in CAF derived from tumours. Exposure of primary normal human fibroblasts to TGF-β1 resulted in the acquisition of a myofibroblastic CAF-like phenotype. This was associated with increased expression of miR-145, a miRNA predicted in silico to target multiple components of the TGF-β signaling pathway. miR-145 was also over-expressed in CAF derived from oral cancers. Over-expression of miR-145 blocked TGF-β1-induced myofibroblastic differentiation and revert CAF towards a normal fibroblast phenotype.We conclude that miR-145 is a key regulator of the CAF phenotype, acting in a negative feedback loop to dampen acquisition of myofibroblastic traits, a key feature of CAF associated with poor disease outcome.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is a pre-copyedited, author-produced version of an article accepted for publication in Carcinogenesis following peer review. The version of record E Melling, SE Flannery, SA Abidin, H Clemmens, P Prajapati, EE Hinsley, S Hunt, JWF Catto, RD Coletta, M Mellone, GJ Thomas, EK Parkinson, SS Prime, IC Paterson, DJ Buttle, DW Lambert; A miRNA-145/TGF-β1 Negative Feedback Loop Regulates the Cancer-Associated Fibroblast Phenotype, Carcinogenesis, , bgy032 is available online at: https://doi.org/10.1093/carcin/bgy032 |
Keywords: | miR-145; myofibroblast; CAF; microRNA |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Clinical Dentistry (Sheffield) The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 13 Mar 2018 09:24 |
Last Modified: | 12 Oct 2020 10:28 |
Status: | Published |
Publisher: | Oxford University Press |
Refereed: | Yes |
Identification Number: | 10.1093/carcin/bgy032 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:128353 |