Zeqiraj, E orcid.org/0000-0003-0239-5926, Tian, L, Piggott, CA et al. (12 more authors) (2015) Higher-Order Assembly of BRCC36-KIAA0157 Is Required for DUB Activity and Biological Function. Molecular Cell, 59 (6). pp. 970-983. ISSN 1097-2765
Abstract
BRCC36 is a Zn²⁺-dependent deubiquitinating enzyme (DUB) that hydrolyzes lysine-63-linked ubiquitin chains as part of distinct macromolecular complexes that participate in either interferon signaling or DNA-damage recognition. The MPN⁺ domain protein BRCC36 associates with pseudo DUB MPN⁻ proteins KIAA0157 or Abraxas, which are essential for BRCC36 enzymatic activity. To understand the basis for BRCC36 regulation, we have solved the structure of an active BRCC36-KIAA0157 heterodimer and an inactive BRCC36 homodimer. Structural and functional characterizations show how BRCC36 is switched to an active conformation by contacts with KIAA0157. Higher-order association of BRCC36 and KIAA0157 into a dimer of heterodimers (super dimers) was required for DUB activity and interaction with targeting proteins SHMT2 and RAP80. These data provide an explanation of how an inactive pseudo DUB allosterically activates a cognate DUB partner and implicates super dimerization as a new regulatory mechanism underlying BRCC36 DUB activity, subcellular localization, and biological function.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Animals; Ants; Catalytic Domain; Crystallography, X-Ray; HEK293 Cells; HeLa Cells; Humans; Insect Proteins; Kinetics; Membrane Proteins; Models, Molecular; Nuclear Matrix-Associated Proteins; Protein Binding; Protein Multimerization; Protein Structure, Quaternary; Protein Structure, Secondary; Ubiquitin-Specific Proteases |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Mar 2018 16:26 |
Last Modified: | 25 Jun 2023 21:15 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.molcel.2015.07.028 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:127916 |