Thomson, E.E., Heuer, A., Schmidt, C. et al. (4 more authors) (2017) Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae. eLife, 6. e30189. ISSN 2050-084X
Abstract
Mechanistic understanding of eukaryotic ribosome formation requires a detailed structural knowledge of the numerous assembly intermediates, generated along a complex pathway. Here, we present the structure of a late pre-40S particle at 3.6 Å resolution, revealing in molecular detail how assembly factors regulate the timely folding of pre-18S rRNA. The structure shows that, rather than sterically blocking 40S translational active sites, the associated assembly factors Tsr1, Enp1, Rio2 and Pno1 collectively preclude their final maturation, thereby preventing untimely tRNA and mRNA binding and error prone translation. Moreover, the structure explains how Pno1 coordinates the 3'end cleavage of the 18S rRNA by Nob1 and how the late factor's removal in the cytoplasm ensures the structural integrity of the maturing 40S subunit.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017, Heuer et al. This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 21 Dec 2017 11:54 |
Last Modified: | 13 Jan 2020 14:29 |
Published Version: | https://doi.org/10.7554/eLife.30189 |
Status: | Published |
Publisher: | eLife Sciences Publications Ltd |
Refereed: | Yes |
Identification Number: | 10.7554/eLife.30189 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:125117 |