White Rose University Consortium logo
University of Leeds logo University of Sheffield logo York University logo

Toxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding

Robinson, S.A., Smith, J.E. and Millner, P.A. (2004) Toxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding. Parasitology, 128 (4). pp. 391-396. ISSN 0031-1820

Full text available as:
[img]
Preview
Text
millner1.pdf
Available under licence : See the attached licence file.

Download (116Kb)

Abstract

Previous studies have indicated that SAG1, the major surface molecule of the protozoan parasite Toxoplasma gondii, is an important attachment ligand for the host cell. However, the research data that supports this claim comes largely from studies investigating tachyzoite binding, and not SAG1 binding per se.

In this study we successfully developed an in vitro attachment assay to directly evaluate the mechanism of SAG1-host cell binding. Competition experiments were then performed using SAG1 that had been pre-treated with the neoglycoprotein BSA-glucosamide or with antibody. Soluble BSA-glucosamide blocked SAG1 attachment to MDBK cells in a dose-dependent manner, implying that SAG1 binding is mediated, in part, via attachment to host cell surface glucosamine. Interestingly, pre-incubation of SAG1 in polyclonal sera from chronically infected mice failed to block binding. This challenges the assumption that anti-SAG1 antibodies block parasite attachment through the masking of SAG1 host cell binding domains. Taken together, this evidence presents new strategies for understanding SAG1-mediated attachment.

Item Type: Article
Copyright, Publisher and Additional Information: © Cambridge University Press
Keywords: Toxoplasma gondii, attachment, SAG1, glucosamide, antibody
Institution: The University of Leeds
Academic Units: The University of Leeds > Faculty of Biological Sciences (Leeds) > Institute of Integrative and Comparative Biology (Leeds)
The University of Leeds > Faculty of Biological Sciences (Leeds) > Institute of Membrane and Systems Biology (Leeds)
Depositing User: Repository Assistant
Date Deposited: 05 Jun 2006
Last Modified: 07 Jun 2014 02:07
Published Version: http://dx.doi.org/10.1017/S0031182003004736
Status: Published
Publisher: Cambridge University Press
Refereed: Yes
Identification Number: 10.1017/S0031182003004736
URI: http://eprints.whiterose.ac.uk/id/eprint/1245

Actions (login required)

View Item View Item