Wilkinson, K.A., Martin, S., Tyagarajan, S.K. et al. (6 more authors) (2017) Commentary: Analysis of SUMO1-conjugation at synapses. Frontiers in Cellular Neuroscience, 11. 345. ISSN 1662-5102
Abstract
A commentary on Analysis of SUMO1-conjugation at synapses
by Daniel, J. A., Cooper, B. H., Palvimo, J. J., Zhang, F. P., Brose, N., and Tirard, M. (2017). eLife 6:e26338. doi: 10.7554/eLife.26338
There is a large and growing literature on protein SUMOylation in neurons and other cell types. While there is a consensus that most protein SUMOylation occurs within the nucleus, SUMOylation of many classes of extranuclear proteins has been identified and, importantly, functionally validated. Notably, in neurons these include neurotransmitter receptors, transporters, sodium and potassium channels, mitochondrial proteins, and numerous key pre- and post-synaptic proteins (for reviews see Martin et al., 2007b; Scheschonka et al., 2007; Craig and Henley, 2012; Luo et al., 2013; Guo and Henley, 2014; Henley et al., 2014; Wasik and Filipek, 2014; Peng et al., 2016; Schorova and Martin, 2016; Wu et al., 2016). Furthermore, several groups have reported SUMO1-ylated proteins in synaptic fractions using biochemical subcellular fractionation approaches, using a range of different validated anti-SUMO1 antibodies (Martin et al., 2007a; Feligioni et al., 2009; Loriol et al., 2012; Luo et al., 2013; Marcelli et al., 2017) and many studies have independently observed colocalization of SUMO1 immunoreactivity with synaptic markers (Martin et al., 2007a; Konopacki et al., 2011; Gwizdek et al., 2013; Jaafari et al., 2013; Hasegawa et al., 2014; Ghosh et al., 2016). Tirard and co-workers (Daniel et al., 2017) directly challenge this wealth of compelling evidence. Primarily using a His6-HA-SUMO1 knock-in (KI) mouse, the authors contest any significant involvement of post-translational modification by SUMO1 in the function of synaptic proteins.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Wilkinson, Martin, Tyagarajan, Arancio, Craig, Guo, Fraser, Goldstein and Henley. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | SUMO1; synapse; sumoylation; neurons; post-translational modification |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 15 Nov 2017 11:21 |
Last Modified: | 29 Oct 2018 14:43 |
Published Version: | https://doi.org/10.3389/fncel.2017.00345 |
Status: | Published |
Publisher: | Frontiers Media |
Refereed: | Yes |
Identification Number: | 10.3389/fncel.2017.00345 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:123957 |