Bose, D.A. orcid.org/0000-0002-0276-6486, Donahue, G., Reinberg, D. et al. (3 more authors) (2017) RNA Binding to CBP Stimulates Histone Acetylation and Transcription. Cell, 168 (1-2). 135-149.e22. ISSN 0092-8674
Abstract
CBP/p300 are transcription co-activators whose binding is a signature of enhancers, cis-regulatory elements that control patterns of gene expression in multicellular organisms. Active enhancers produce bi-directional enhancer RNAs (eRNAs) and display CBP/p300-dependent histone acetylation. Here, we demonstrate that CBP binds directly to RNAs in vivo and in vitro. RNAs bound to CBP in vivo include a large number of eRNAs. Using steady-state histone acetyltransferase (HAT) assays, we show that an RNA binding region in the HAT domain of CBP—a regulatory motif unique to CBP/p300—allows RNA to stimulate CBP’s HAT activity. At enhancers where CBP interacts with eRNAs, stimulation manifests in RNA-dependent changes in the histone acetylation mediated by CBP, such as H3K27ac, and by corresponding changes in gene expression. By interacting directly with CBP, eRNAs contribute to the unique chromatin structure at active enhancers, which, in turn, is required for regulation of target genes.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Elsevier. This is an author produced version of a paper subsequently published in Cell. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/) |
Keywords: | gene regulation; chromatin modification; histone acetylation; CBP/p300; acetyltransferase; epigenetic enzymes; non-coding RNA; RNA-binding; enhancers; eRNA |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 01 Jun 2017 14:40 |
Last Modified: | 30 Apr 2020 12:54 |
Published Version: | https://doi.org/10.1016/j.cell.2016.12.020 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.cell.2016.12.020 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:117190 |
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