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Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis

Davison, P.A., Hunter, C.N. and Horton, P. (2002) Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis. Nature, 418 (6894). pp. 203-206. ISSN 0028-0836

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Abstract

Plant stress caused by extreme environmental conditions is already a principal reason for yield reduction in crops. The threat of global environment change makes it increasingly important to generate crop plants that will withstand such conditions. Stress, particularly stress caused by increased sunlight, leads to the production of reactive oxygen species that cause photo-oxidative cell damage. Carotenoids, which are present in the membranes of all photosynthetic organisms, help protect against such light-dependent oxidative damage. In plants, the xanthophyll cycle (the reversible interconversion of two carotenoids, violaxanthin and zeaxanthin) has a key photoprotective role and is therefore a promising target for genetic engineering to enhance stress tolerance. Here we show that in Arabidopsis thaliana overexpression of the chyB gene that encodes -carotene hydroxylase—an enzyme in the zeaxanthin biosynthetic pathway—causes a specific twofold increase in the size of the xanthophyll cycle pool. The plants are more tolerant to conditions of high light and high temperature, as shown by reduced leaf necrosis, reduced production of the stress indicator anthocyanin and reduced lipid peroxidation. Stress protection is probably due to the function of zeaxanthin in preventing oxidative damage of membranes.

Item Type: Article
Copyright, Publisher and Additional Information: © 2002 Nature Publishing Group
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield)
The University of Sheffield > University of Sheffield Research Centres and Institutes > Robert Hill Institute (Sheffield)
Depositing User: Repository Officer
Date Deposited: 20 Sep 2004
Last Modified: 08 Feb 2013 16:46
Published Version: http://www.nature.com/nature
Status: Published
Refereed: Yes
Identification Number: 10.1038/nature00861
URI: http://eprints.whiterose.ac.uk/id/eprint/110

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