Williams, JM, Bezak, T, Das, M et al. (9 more authors) (2016) Psg22 null mouse embryos develop normally under normoxic and hypoxic conditions of pregnancy. Science Matters.
Abstract
Pregnancy-specific glycoproteins are secreted immunoglobulin superfamily members encoded by multigene families in eutherian mammals with haemochorial placentation. They are expressed predominantly in placental trophoblast and exhibits immunomodulatory, anti-platelet and pro-angiogenic functions. An inversion of Psg22 in the mouse locus is associated with relatively high Psg22 expression in the first half of the pregnancy. Bioinformatic analysis of seventeen mouse strains indicated that the Psg22 inversion arose at least 1.7 MYA. We used CRISPR-Cas9 mutagenesis to generate Psg22 null mutants, two of which were analysed in detail (Psg22Δ10 and Psg22Δ16). Both mutants contain frame-shifting deletions in exon 2, resulting in premature stop codons, and Psg22 mRNA was virtually undetectable. Both mutants are fertile and there was no distortion of Mendelian ratios in heterozygous crosses. Housing of pregnant females in a hypoxic (11% O2) environment for five (E5 - E10) or ten (E5 - E15) days did not induce differential growth or survival of Psg22 wildtype and null mutant genotypes. Our results indicate that Psg22 is dispensable for embryonic development and reproduction under laboratory conditions. As PSGs are secreted into maternal blood, future work will focus on whether Psg22 deficiency alters maternal physiology.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Pregnancy Specific Glycoprotein 22; Adaptation Physiological; Mice Knockout; Pregnancy; Stress Physiological |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Jan 2017 10:59 |
Last Modified: | 12 Aug 2019 16:27 |
Published Version: | http://doi.org/10.19185/matters.201611000023 |
Status: | Published |
Publisher: | University of Zurich |
Identification Number: | 10.19185/matters.201611000023 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:109983 |