Rapid Construction of Recombinant Plasmids by QuickStep-Cloning

Jajesniak, P. and Wong, T.S. (2016) Rapid Construction of Recombinant Plasmids by QuickStep-Cloning. Methods in Molecular Biology, 1472. pp. 205-214. ISSN 1940-6029

Abstract

Metadata

Authors/Creators:
  • Jajesniak, P.
  • Wong, T.S.
Copyright, Publisher and Additional Information: © 2016 Springer Science+Business Media New York. This is an author produced version of a paper subsequently published in Methods in Molecular Biology. Uploaded in accordance with the publisher's self-archiving policy.
Keywords: Recombinant DNA; Megaprimer PCR; Ligation-independent cloning; Plasmid construction; Synthetic biology; Protein engineering; Metabolic engineering
Dates:
  • Published: 27 September 2016
  • Accepted: 17 June 2016
  • Published (online): 27 September 2016
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield)
Depositing User: Symplectic Sheffield
Date Deposited: 03 Jan 2017 11:09
Last Modified: 27 Sep 2017 20:07
Published Version: https://doi.org/10.1007/978-1-4939-6343-0_16
Status: Published
Publisher: Springer Verlag
Refereed: Yes
Identification Number: https://doi.org/10.1007/978-1-4939-6343-0_16

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