Neumann, Y., Bruns, S.A., Rohde, M. et al. (3 more authors) (2016) Intracellular Staphylococcus aureus eludes selective autophagy by activating a host cell kinase. Autophagy, 12 (11). pp. 2069-2084. ISSN 1554-8627
Abstract
Autophagy, a catabolic pathway of lysosomal degradation, acts not only as an efficient recycle and survival mechanism during cellular stress, but also as an anti-infective machinery. The human pathogen Staphylococcus aureus (S. aureus) was originally considered solely as an extracellular bacterium, but is now recognized additionally to invade host cells, which might be crucial for persistence. However, the intracellular fate of S. aureus is incompletely understood. Here, we show for the first time induction of selective autophagy by S. aureus infection, its escape from autophagosomes and proliferation in the cytoplasm using live cell imaging. After invasion, S. aureus becomes ubiquitinated and recognized by receptor proteins such as SQSTM1/p62 leading to phagophore recruitment. Yet, S. aureus evades phagophores and prevents further degradation by a MAPK14/p38a MAP kinase-mediated blockade of autophagy. Our study demonstrates a novel bacterial strategy to block autophagy and secure survival inside the host cell.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Taylor & Francis. |
Keywords: | ATG5; MAP kinase14 S. aureus; selective autophagy; ubiquitin |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Funding Information: | Funder Grant number EUROPEAN COMMISSION - FP6/FP7 LSHM-CT-2006-019064 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 07 Dec 2016 14:41 |
Last Modified: | 07 Dec 2016 14:41 |
Published Version: | http://doi.org/10.1080/15548627.2016.1226732 |
Status: | Published |
Publisher: | Taylor & Francis |
Refereed: | Yes |
Identification Number: | 10.1080/15548627.2016.1226732 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:109045 |