Jing, J., Junutula, J. R., Wu, C. et al. (4 more authors) (2010) FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network. Molecular Biology of the Cell, 21 (17). pp. 3041-3053. ISSN 1059-1524
Abstract
Many proteins are retrieved to the trans-Golgi Network (TGN) from the endosomal system through several retrograde transport pathways to maintain the composition and function of the TGN. However, the molecular mechanisms involved in these distinct retrograde pathways remain to be fully understood. Here we have used fluorescence and electron microscopy as well as various functional transport assays to show that Rab11a/b and its binding protein FIP1/RCP are both required for the retrograde delivery of TGN38 and Shiga toxin from early/recycling endosomes to the TGN, but not for the retrieval of mannose-6-phosphate receptor from late endosomes. Furthermore, by proteomic analysis we identified Golgin-97 as a FIP1/RCP-binding protein. The FIP1/RCP-binding domain maps to the C-terminus of Golgin-97, adjacent to its GRIP domain. Binding of FIP1/RCP to Golgin-97 does not affect Golgin-97 recruitment to the TGN, but appears to regulate the targeting of retrograde transport vesicles to the TGN. Thus, we propose that FIP1/RCP binding to Golgin-97 is required for tethering and fusion of recycling endosome-derived retrograde transport vesicles to the TGN.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2010 J. Jing et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-ncsa/3.0). |
Keywords: | Adaptor Proteins, Signal Transducing; Adaptor Proteins, Vesicular Transport; Autoantigens; Endocytosis; Endosomes; Gene Knockdown Techniques; Green Fluorescent Proteins; HeLa Cells; Humans; Membrane Glycoproteins; Membrane Proteins; Models, Biological; Protein Binding; Protein Interaction Mapping; Protein Transport; Receptors, Transferrin; Recombinant Fusion Proteins; Shiga Toxin; rab GTP-Binding Proteins; trans-Golgi Network |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 21 Dec 2016 15:26 |
Last Modified: | 21 Dec 2016 15:28 |
Published Version: | http://dx.doi.org/10.1091/mbc.E10-04-0313 |
Status: | Published |
Publisher: | American Society for Cell Biology |
Refereed: | Yes |
Identification Number: | 10.1091/mbc.E10-04-0313 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108295 |