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Raman spectroscopy and advanced mathematical modelling in the discrimination of human thyroid cell lines

Harris, A.T., Garg, M., Yang, X.B., Fisher, S.E., Kirkham, J., Smith, A., Martin-Hirsch, D.P. and High, A.S. (2009) Raman spectroscopy and advanced mathematical modelling in the discrimination of human thyroid cell lines. Head & Neck Oncology , 1. ISSN 1472-6963

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Abstract

Raman spectroscopy could offer non-invasive, rapid and an objective nature to cancer diagnostics. However, much work in this field has focused on resolving differences between cancerous and non-cancerous tissues, and lacks the reproducibility and interpretation to be put into clinical practice. Much work is needed on basic cellular differences between malignancy and normal. This would allow the establishment of a clinically relevant cellular based model to translate to tissue classification. Raman spectroscopy provides a very detailed biochemical analysis of the target material and to 'unlock' this potential requires sophisticated mathematical modelling such as neural networks as an adjunct to data interpretation. Commercially obtained cancerous and non-cancerous cells, cultured in the laboratory were used in Raman spectral measurements. Data trends were visualised through PCA and then subjected to neural network analysis based on self-organising maps; consisting of m maps, where m is the number of classes to be recognised. Each map approximates the statistical distribution of a given class. The neural network analysis provided a 95% accuracy for identification of the cancerous cell line and 92% accuracy for normal cell line. In this preliminay study we have demonstrated th ability to distinguish between "normal" and cancerous commercial cell lines. This encourages future work to establish the reasons underpinning these spectral differences and to move forward to more complex systems involving tissues. We have also shown that the use of sophisticated mathematical modelling allows a high degree of discrimination of 'raw' spectral data.

Item Type: Article
Copyright, Publisher and Additional Information: © 2009 Harris et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Institution: The University of Leeds
Academic Units: The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Pathology (Leeds) > Pathology (Leeds)
The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds)
Depositing User: Sherpa Assistant
Date Deposited: 06 May 2010 09:34
Last Modified: 08 Feb 2013 17:07
Published Version: http://dx.doi.org/10.1186/1758-3284-1-38
Status: Published
Publisher: Biomed Central
Identification Number: 10.1186/1758-3284-1-38
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/10787

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