Katerinaki, E., Evans, G.S., Lorigan, P.C. et al. (1 more author) (2003) TNF-alpha increases human melanoma cell invasion and migration in vitro: the role of proteolytic enzymes. British Journal of Cancer, 89 (6). pp. 1123-1129. ISSN 0007-0920
Abstract
Inflammatory mediators have been reported to promote malignant cell growth, invasion and metastatic potential. More specifically, we have recently reported that tumour necrosis factor alpha (TNF-a) increases melanoma cell attachment to extracellular matrix (ECM) substrates and invasion through fibronectin. In this study, we extend these investigations asking specifically whether the TNF-a effect on cell invasion and migration involves activation of proteolytic enzymes. We examined the effect of TNF-a on melanoma expression/activation of type IV gelatinases matrix metalloproteinases 2 and 9 (MMPs -2 and -9) and general proteolytic enzymes. Stimulation with TNF-a significantly increased both melanoma cell migration at 24 h ( þ 21%) and invasion through fibronectin ( þ 35%) but did not upregulate/activate the expression of latent MMP-2 constitutively produced by these cells and did not upregulate their general protease activity. However, the increased cell migration and invasion through fibronectin observed following stimulation with TNF-a were inhibited by the general protease inhibitor a2 macroglobulin. These findings suggest that the promigratory and proinvasive effect of TNF-a on this melanoma cell line may be mediated to some extent by induction of localised cell membrane-bound degradative enzyme activity, which is not readily detected in biochemical assays.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2003 Cancer Research UK. From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
Keywords: | Melanoma; Invasion; Migration; Tnf-Alpha; Mmp-2; Degradative Enzymes; Tumor-Necrosis-Factor; Stimulating Hormone; Expression; Progression; Fibronectin; Melanocytes; Modulation; Integrins; Receptors; Motility |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Dec 2016 15:39 |
Last Modified: | 08 Dec 2016 15:56 |
Published Version: | https://doi.org/10.1038/sj.bjc.6601257 |
Status: | Published |
Publisher: | Cancer Research UK |
Refereed: | Yes |
Identification Number: | 10.1038/sj.bjc.6601257 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:107757 |
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Licence: CC-BY-NC-SA 3.0