Marshall, J., Rossez, Y., Mainda, G. et al. (3 more authors) (2016) Alternate thermoregulation and functional binding of Escherichia coli Type 1 fimbriae in environmental and animal isolates. FEMS Microbiology Letters. ISSN 0378-1097
Abstract
Type 1 fimbriae (T1F) are well characterised cell surface organelles expressed by Escherichia coli and required for adherence to mannosylated host tissue. They satisfy molecular Koch's postulates as a virulence determinant and a host-adapted role has been reinforced by reports that T1F expression is repressed at sub-mammalian temperatures. Analysis of a group of 136 environmental and animal E. coli isolates that express T1F at 37 °C showed that 28% are also capable of expression at 20 °C, in a phase variable manner. The heterogeneous proportions varied widely, and although growth temperature impacted the total proportion expressing T1F, there was no direct correlation between growth at 37 and 20 °C, indicative of differences in thermoregulation of the genetic switch (fimS) that controls phase variation. Specificities of the adhesin (FimH) also varied between the isolates: most bound to α-(1-3) mannan and yeast extracts as expected, but some recognised β-(1-4)-mannans and N-linked glycoproteins from plants, and T1F from two of the isolates mediated binding to plant roots. The results expand our view of a well described adherence factor to show alternative expression profiles and adhesin specificities, which in turn may confer an advantage for certain isolates in alternative hosts and habitats.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 FEMS. This is an author produced version of a paper subsequently published in FEMS Microbiology Letters. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | adhesin; pili; mannosides; plants; animals; T-RLFP |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Nov 2016 15:34 |
Last Modified: | 02 Nov 2017 01:38 |
Published Version: | https://doi.org/10.1093/femsle/fnw251 |
Status: | Published |
Publisher: | Oxford University Press |
Refereed: | Yes |
Identification Number: | 10.1093/femsle/fnw251 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:106808 |