Messad, N., Prajsnar, T.K., Lina, G. et al. (9 more authors) (2015) Existence of a Colonizing Staphylococcus aureus Strain Isolated in Diabetic Foot Ulcers. Diabetes, 64 (8). pp. 2991-2995. ISSN 0012-1797
Abstract
Staphylococcus aureus is an opportunistic bacterium capable of causing a wide range of severe diseases when it gains access to underlying tissues. Paradoxically, S. aureus is a common inhabitant of the skin microflora and colonizes the nares and other human mucosa. The purpose of this study was to determine the genetic basis for the differences in the pathogenic versus colonizing potential of S. aureus isolated from diabetic foot ulcers (DFUs). By performing optical map comparisons of a collection of S. aureus strains isolated from DFUs, we brought to light a prophage present in noninfecting bacteria. The phage, namely ROSA-like, was localized in a hotspot region ΦNM2 near the locus isd, the iron surface determinant system. The integrated phage significantly reduces the virulence of the strain and increases the biofilm formation. DFUs seem to be a specific niche of this colonizing strain. The ROSA-like phage represents the first description of a mobile element present mainly in S. aureus isolated from DFUs, which modulates the relationship of the bacteria with its human host. This phage appears to attenuate bacterial virulence and promote colonization.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 American Diabetes Association. This is an author produced version of a paper subsequently published in Diabetes. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Funding Information: | Funder Grant number BRITISH INFECTION ASSOCIATION None WELLCOME TRUST (THE) 089981/Z/09/Z EUROPEAN COMMISSION - FP6/FP7 FISH4PHARMA - 289209 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 07 Oct 2016 15:22 |
Last Modified: | 04 Nov 2016 04:43 |
Published Version: | http://dx.doi.org/10.2337/db15-0031 |
Status: | Published |
Publisher: | American Diabetes Association |
Refereed: | Yes |
Identification Number: | 10.2337/db15-0031 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:105676 |