Bridgewood, C, Stacey, M orcid.org/0000-0003-3502-5542, Alase, A et al. (3 more authors) (2017) IL-36γ has proinflammatory effects on human endothelial cells. Experimental Dermatology, 26 (5). pp. 402-408. ISSN 0906-6705
Abstract
Interleukin-36 cytokines are predominantly expressed by epithelial cells. Significant upregulation of epidermal IL-36 is now a recognised characteristic of psoriatic skin inflammation. IL-36 is known to induce inflammatory responses in dendritic cells, fibroblasts and epithelial cells. Although vascular alterations are a hallmark of psoriatic lesions and dermal endothelial cells are well known to play a critical role in skin inflammation, the effects of IL-36 on endothelial cells are unexplored. We here show that endothelial cells including dermal microvascular cells express a functionally active IL-36 receptor. Adhesion molecules VCAM-1 and ICAM-1 are upregulated by IL-36γ stimulation and this is reversed by the presence of the endogenous IL-36 receptor antagonist. IL-36γ stimulated endothelial cells secrete the proinflammatory chemokines IL-8, CCL2 and CCL20. Chemotaxis assays showed increased migration of T cells following IL-36γ stimulation of endothelial cells. These results suggest a role for IL-36γ in the dermal vascular compartment and it is likely to enhance psoriatic skin inflammation by activating endothelial cells and promoting leukocyte recruitment.
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Copyright, Publisher and Additional Information: | © 2016 John Wiley & Sons A/S. This is the peer reviewed version of the following article: Bridgewood, C., Stacey, M., Alase, A., Lagos, D., Graham, A. and Wittmann, M. (2016), IL-36γ has proinflammatory effects on human endothelial cells. Exp Dermatol. Accepted Author Manuscript. doi:10.1111/exd.13228, which has been published at http://dx.doi.org/10.1111/exd.13228. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | ||||||
Keywords: | Psoriasis, endothelial, skin, inflammation, IL-36 | ||||||
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Institution: | The University of Leeds | ||||||
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) | ||||||
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Depositing User: | Symplectic Publications | ||||||
Date Deposited: | 29 Sep 2016 10:49 | ||||||
Last Modified: | 07 Dec 2017 16:29 | ||||||
Published Version: | http://dx.doi.org/10.1111/exd.13228 | ||||||
Status: | Published | ||||||
Publisher: | Wiley | ||||||
Identification Number: | https://doi.org/10.1111/exd.13228 |