Blakeston, A.C., Alswieleh, A.M., Heath, G.R. et al. (7 more authors) (2015) New Poly(amino acid methacrylate) Brush Supports the Formation of Well-Defined Lipid Membranes. Langmuir, 31 (12). pp. 3668-3677. ISSN 0743-7463
Abstract
A novel poly(amino acid methacrylate) brush comprising zwitterionic cysteine groups (PCysMA) was utilized as a support for lipid bilayers. The polymer brush provides a 12-nm-thick cushion between the underlying hard support and the aqueous phase. At neutral pH, the zeta potential of the PCysMA brush was ∼−10 mV. Cationic vesicles containing >25% DOTAP were found to form a homogeneous lipid bilayer, as determined by a combination of surface analytical techniques. The lipid mobility as measured by FRAP (fluorescence recovery after photobleaching) gave diffusion coefficients of ∼1.5 μm2 s–1, which are comparable to those observed for lipid bilayers on glass substrates.
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Copyright, Publisher and Additional Information: | © 2015 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. | ||||
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Institution: | The University of Sheffield | ||||
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) | ||||
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Depositing User: | Symplectic Sheffield | ||||
Date Deposited: | 22 Sep 2016 14:27 | ||||
Last Modified: | 22 Sep 2016 14:27 | ||||
Published Version: | https://dx.doi.org/10.1021/la504163s | ||||
Status: | Published | ||||
Publisher: | American Chemical Society | ||||
Refereed: | Yes | ||||
Identification Number: | https://doi.org/10.1021/la504163s | ||||
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