Bloor, D.J., Wilson, Y., Kibschull, M., Traub, O., Winterhager, E., Kimber, S.J. and Leese, H.J. (2004) Expression of connexins in human preimplantation embryos in vitro. Reproductive Biology and Endocrinology. ISSN 1477-7827Full text available as:
Intercellular communication via gap junctions is required to coordinate developmental processes in the mammalian embryo. We have investigated if the connexin (Cx) isoforms known to form gap junctions in rodent preimplantation embryos are also expressed in human embryos, with the aim of identifying species differences in communication patterns in early development. Using a combination of polyA PCR and immunocytochemistry we have assessed the expression of Cx26, Cx31, Cx32, Cx40, Cx43 and Cx45 which are thought to be important in early rodent embryos. The results demonstrate that Cx31 and Cx43 are the main connexin isoforms expressed in human preimplantation embryos and that these isoforms are co-expressed in the blastocyst. Cx45 protein is expressed in the blastocyst but the protein may be translated from a generally low level of transcripts: which could only be detected in the PN to 4-cell embryos. Interestingly, Cx40, which is expressed by the extravillous trophoblast in the early human placenta, was not found to be expressed in the blastocyst trophectoderm from which this tissue develops. All of the connexin isoforms in human preimplantation embryos are also found in rodents pointing to a common regulation of these connexins in development of rodent and human early embryos and perhaps other species.
|Copyright, Publisher and Additional Information:||© 2004 Bloor et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.|
|Academic Units:||The University of York > Biology (York)|
|Depositing User:||Repository Officer|
|Date Deposited:||17 Feb 2006|
|Last Modified:||17 Oct 2013 14:26|