Munck, J.M., Motley, A.M., Nuttall, J.M. and Hettema, E.H. (2009) A dual function for Pex3p in peroxisome formation and inheritance. Journal of Cell Biology, 187 (4). pp. 463-471. ISSN 0021-9525
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Abstract
Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show that the peroxisome retention factor Inp1p interacts physically with Pex3p in vitro and in vivo, and split-GFP analysis shows that the site of interaction is the peroxisomal membrane. Furthermore, we have generated PEX3 alleles that support peroxisome formation but fail to support recruitment of Inp1p to peroxisomes, and as a consequence are affected in peroxisome segregation. We conclude that Pex3p functions as an anchor for Inp1p at the peroxisomal membrane, and that this function is independent of its role at the ER in peroxisome biogenesis.
| Item Type: | Article |
|---|---|
| Copyright, Publisher and Additional Information: | © 2009 Munck et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
| Keywords: | Protein-protein Interactions; Saccharomyces-Cerevisiae; Endoplasmic-Reticulum; Membrane-Proteins; Cell-Division; Yeast; Biogenesis; Import; MYO2P; Mitochondria |
| Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
| Depositing User: | Miss Anthea Tucker |
| Date Deposited: | 02 Dec 2009 16:48 |
| Last Modified: | 08 Feb 2013 16:59 |
| Published Version: | http://dx.doi.org/10.1083/jcb.200906161 |
| Status: | Published |
| Publisher: | Rockefeller University Press |
| Refereed: | Yes |
| Identification Number: | 10.1083/jcb.200906161 |
| URI: | http://eprints.whiterose.ac.uk/id/eprint/10225 |
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