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The native architecture of a photosynthetic membrane

Bahatyrova, S., Frese, R.N., Siebert, A., Olsen, J.D., van der Werf, K.O., van Grondelle, R., Niederman, R.A., Bullough, P.A., Otto, C. and Hunter, C.N. (2004) The native architecture of a photosynthetic membrane. Nature, 430 (7003). pp. 1058-1062. ISSN 0028-0836

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Abstract

In photosynthesis, the harvesting of solar energy and its subsequent conversion into a stable charge separation are dependent upon an interconnected macromolecular network of membrane-associated chlorophyll–protein complexes. Although the detailed structure of each complex has been determined, the size and organization of this network are unknown. Here we show the use of atomic force microscopy to directly reveal a native bacterial photosynthetic membrane. This first view of any multi-component membrane shows the relative positions and associations of the photosynthetic complexes and reveals crucial new features of the organization of the network: we found that the membrane is divided into specialized domains each with a different network organization and in which one type of complex predominates. Two types of organization were found for the peripheral light-harvesting LH2 complex. In the first, groups of 10–20 molecules of LH2 form light-capture domains that interconnect linear arrays of dimers of core reaction centre (RC)–light-harvesting 1 (RC–LH1–PufX) complexes; in the second they were found outside these arrays in larger clusters. The LH1 complex is ideally positioned to function as an energy collection hub, temporarily storing it before transfer to the RC where photochemistry occurs: the elegant economy of the photosynthetic membrane is demonstrated by the close packing of these linear arrays, which are often only separated by narrow 'energy conduits' of LH2 just two or three complexes wide.

Item Type: Article
Copyright, Publisher and Additional Information: © 2004 Nature Publishing Group
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield)
Depositing User: Repository Officer
Date Deposited: 20 Sep 2004
Last Modified: 06 Jun 2014 16:37
Published Version: http://www.nature.com/nature
Status: Published
Refereed: Yes
Identification Number: 10.1038/nature02823
URI: http://eprints.whiterose.ac.uk/id/eprint/102

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