Levenstein, M.A., Robertson, K., Turner, T.D. et al. (9 more authors) (2022) Serial small- and wide-angle X-ray scattering with laboratory sources. IUCrJ, 9 (5). pp. 538-543. ISSN 2052-2525
Abstract
Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology.
Metadata
Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0) | ||||||
Keywords: | serial SAXS; serial WAXS; rapid structural analysis; crystallization; microfluidics | ||||||
Dates: |
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Institution: | The University of Sheffield | ||||||
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) | ||||||
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Depositing User: | Symplectic Sheffield | ||||||
Date Deposited: | 11 Oct 2022 14:02 | ||||||
Last Modified: | 11 Oct 2022 14:02 | ||||||
Status: | Published | ||||||
Publisher: | International Union of Crystallography (IUCr) | ||||||
Refereed: | Yes | ||||||
Identification Number: | https://doi.org/10.1107/s2052252522007631 | ||||||
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