On the roles of AA15 lytic polysaccharide monooxygenases derived from the termite Coptotermes gestroi

Abstract

Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes which catalyze the oxidative cleavage of polysaccharides. LPMOs belonging to family 15 in the Auxiliary Activity (AA) class from the Carbohydrate-Active Enzyme database are found widespread across the Tree of Life, including viruses, algae, oomycetes and animals. Recently, two AA15s from the firebrat Thermobia domestica were reported to have oxidative activity, one towards cellulose or chitin and the other towards chitin, signalling that AA15 LPMOs from insects potentially have different biochemical functions. Herein, we report the identification and characterization of two family AA15 members from the lower termite Coptotermes gestroi. Addition of Cu(II) to CgAA15a or CgAA15b had a thermostabilizing effect on both.

Metadata

Authors/Creators:	Walton, Paul Howard https://orcid.org/0000-0002-1152-1480 Lourenco Franco Cairo, Joao Paulo https://orcid.org/0000-0002-4744-3111 Cannella, David Oliveira, Leandro Goncalves, Thiago Rubio, Marcelo Terrasan, Cesar R.F. Tramontina, Robson Mofatto, Luciana S. Carazzolle, Marcelo F. Garcia, Wanius Felby, Claus Damasio, André Squina, Fabio M.
Copyright, Publisher and Additional Information:	Funding Information: We would like to acknowledge professors Luisa Ciano and Gideon Davies as well as Dr. Alessandro Paradisi for supporting on experiments and for comments on the manuscript. We also would like to acknowledge Dr. Magdalena Calusinska for kindly provide the sequences of LPMOs AA15 from termites Cortaritermes sp. and Macrotermes natalensis. This research was supported by grants from Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo - FAPESP (FMS 2015/50590?4; JPLFC 2016/09950?0; 2017/11952?3; WG 2017/17275?3; TAG 2017/16089?1; AD 2015/50612?8 and 2017/22669?0) and Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq. (FMS 428527/2018?3 and 305740/2017?2; WG 422132/2018?7; LCO 442352/2014?0; AD 404654/2018?5 and 304816/2017?5). We would like to acknowledge the MASS facility from Brazilian Biosciences National Laboratory (LNBio ? CNPEM) Campinas, SP, Brazil; the Analytic Center from the Institute of Chemistry from the State University of Campinas, Campinas, SP, Brazil; and the Technology Facility, Department of Biology, University of York, York, United Kingdom. The molecular dynamics simulations were performed at the Center for Scientific Computing (NCC/GridUNESP) of the S?o Paulo State University (UNESP) and CENAPAD-SP (Centro Nacional de Processamento de Alto Desempenho em S?o Paulo), project UNICAMP/FINEP-MCTII. Funding Information: We would like to acknowledge professors Luisa Ciano and Gideon Davies as well as Dr. Alessandro Paradisi for supporting on experiments and for comments on the manuscript. We also would like to acknowledge Dr. Magdalena Calusinska for kindly provide the sequences of LPMOs AA15 from termites Cortaritermes sp. and Macrotermes natalensis. This research was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP ( FMS 2015/50590–4 ; JPLFC 2016/09950–0 ; 2017/11952–3 ; WG 2017/17275–3 ; TAG 2017/16089–1 ; AD 2015/50612–8 and 2017/22669–0 ) and Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq. ( FMS 428527/2018–3 and 305740/2017–2 ; WG 422132/2018–7 ; LCO 442352/2014–0 ; AD 404654/2018–5 and 304816/2017–5 ). We would like to acknowledge the MASS facility from Brazilian Biosciences National Laboratory (LNBio – CNPEM) Campinas, SP, Brazil; the Analytic Center from the Institute of Chemistry from the State University of Campinas, Campinas, SP, Brazil; and the Technology Facility, Department of Biology, University of York, York, United Kingdom. The molecular dynamics simulations were performed at the Center for Scientific Computing (NCC/GridUNESP) of the São Paulo State University (UNESP) and CENAPAD-SP (Centro Nacional de Processamento de Alto Desempenho em São Paulo), project UNICAMP/FINEP-MCTII. Publisher Copyright: © 2020 Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
Keywords:	AA15, CAZymes, Chitin, Chitinases, LPMOs, Termites
Dates:	Accepted: 18 November 2020 Published (online): 23 November 2020 Published: 6 January 2021
Institution:	The University of York
Academic Units:	The University of York > Faculty of Sciences (York) > Chemistry (York)
Depositing User:	Pure (York)
Date Deposited:	07 Apr 2022 08:20
Last Modified:	04 Feb 2024 01:11
Published Version:	https://doi.org/10.1016/j.jinorgbio.2020.111316
Status:	Published
Refereed:	Yes
Identification Number:	https://doi.org/10.1016/j.jinorgbio.2020.111316
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