Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.

Abstract

The fibrin gel angiogenesis bead assay provides a controlled in vitro setting for observing endothelial angiogenic sprouting in response to modified variables. Endothelial cells are coated onto microcarriers and embedded into a fibrin clot containing necessary growth factors. Following a 24-h incubation, endothelial sprouts are imaged using a light microscope. This method is useful for rapidly and affordably investigating the effects of genetic or chemical manipulation to endothelial function.

Metadata

Authors/Creators:

Clavane, EM
Taylor, HA
Cubbon, RM https://orcid.org/0000-0001-7844-3600
Meakin, PJ https://orcid.org/0000-0002-8553-3863

Keywords:

Angiogenesis; Fibrin matrix; Cytodex® 3 beads; Endothelial cells; Sprouting

Dates:

Published: 1 January 2022

Institution:

The University of Leeds

Academic Units:

The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Discovery & Translational Science Dept (Leeds)

Funding Information:

Funder	Grant number
British Heart Foundation	FS/13/38/33659
BBSRC (Biotechnology & Biological Sciences Research Council)	BB/V014358/1

Depositing User:

Symplectic Publications

Date Deposited:

07 Feb 2022 13:35

Last Modified:

07 Feb 2022 13:35

Status:

Published

Publisher:

Springer

Series Name:

Methods in Molecular Biology

Identification Number:

https://doi.org/10.1007/978-1-0716-2059-5_25

Related URLs:

PubMed URL

CORE (COnnecting REpositories)

CORE (COnnecting REpositories)