Xie, X orcid.org/0000-0002-1474-9931, van Delft, MAM, Shuweihdi, F orcid.org/0000-0003-1199-2992 et al. (5 more authors) (2021) Auto-antibodies to post-translationally modified proteins in osteoarthritis. Osteoarthritis and Cartilage, 29 (6). pp. 924-933. ISSN 1063-4584
Abstract
Objective
Autoantibodies (AutoAbs) have been observed in osteoarthritis (OA) with broad antigenicity, although their prevalence and role remain unclear. Post-translational modification (PTMs) of proteins (oxidation, carbamylation, citrullination) is associated with synovitis and can lead to AutoAb development. Given the prevalence of synovitis, we explored whether AutoAbs to PTM-antigens are common in OA compared with rheumatoid arthritis (RA).
Methods
Serum (n = 895) was obtained from healthy controls, OA and RA patients; and arthritic synovial fluid (SF, n = 290). ELISAs were used to quantify anti-citrullinated peptide (ACPA), anti-carbamylated protein (anti-CarP), anti-oxidized collagen (anti-ROS-CI/CII) antibodies.
Results
In sera, positivity for PTM-antigens AutoAbs was observed at a lower frequency in OA with 64.1% (95%CI: 57.2–70.1%) more ACPA+ and 29.8% (21.0–37.3%) more anti-CarP + patients in RA (both P < 0.0001). Levels of ACPA, anti-CarP were also lower in OA (P < 0.0001). Anti-ROS-CII positivity was lower in OA compared to RA (16.6%, 4.8–28.6%) less frequent, P = 0.033) but not anti-native-CII. There was no impact of age/gender on AutoAbs associations with diseases either looking at positivity or levels. In SF, OA patients were often ACPA+ (45.9%) although less frequently than in RA (P = 0.004). Anti-CarP were rarely observed (<5% all samples). All collagen AutoAbs were more frequent in RA compared to OA (all P < 0.010) but only levels of anti-CII and anti-ROS-CII were significantly higher in they RA (P < 0.050).
Conclusion
Although the frequency of AutoAbs for PTM proteins were lower in OA sera compared to RA, a higher proportion of OA SF were positive. The relative retention of AutoAbs in the OA joint requires further investigation.
Metadata
Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. This is an author produced version of an article published in Osteoarthritis and Cartilage. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | PTM; AutoAbs; OA; RA; ACPA; anti-CarP; Anti-ROS-CI; Anti-ROS-CII |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) > Inflammatory Arthritis (Leeds) ?? Leeds.SU-RMMR ?? The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Health Sciences (Leeds) > Centre for Health Services Research (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Jul 2021 13:52 |
Last Modified: | 20 Mar 2022 01:38 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | https://doi.org/10.1016/j.joca.2021.03.008 |
Related URLs: |
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