The characterisation of microsatellite markers reveals tetraploidy in the Greater Water Parsnip, Sium latifolium (Apiaceae).

Abstract

BACKGROUND: The Greater Water Parsnip, Sium latifolium (Apiaceae), is a marginal aquatic perennial currently endangered in England and consequently the focus of a number of conservation translocation projects. Microsatellite markers were developed for S. latifolium to facilitate comparison of genetic diversity and composition between natural and introduced populations.

RESULTS: We selected 65 S. latifolium microsatellite (MiSeq) sequences and designed primer pairs for these. Primer sets were tested in 32 individuals. We found 15 polymorphic loci that amplified consistently. For the selected 15 loci, the number of alleles per locus ranged from 8 to 17. For all loci, S. latifolium individuals displayed up to four alleles indicating polyploidy in this species.

CONCLUSIONS: These are the first microsatellite loci developed for S. latifolium and each individual displayed 1-4 alleles per locus, suggesting polyploidy in this species. These markers provide a valuable resource in evaluating the population genetic composition of this endangered species and thus will be useful for guiding conservation and future translocations of the species.

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Authors/Creators:	Dalton, N.J. https://orcid.org/0000-0001-6269-8721 Horsburgh, G.J. Dawson, D.A.
Copyright, Publisher and Additional Information:	© The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Keywords:	Microsatellite; Plant translocation; Polyploid; Simple sequence repeat (SSR); Simple tandem repeat (STR); Sium latifolium
Dates:	Accepted: 2 June 2017 Published: 12 June 2017
Institution:	The University of Sheffield
Academic Units:	The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield)
Depositing User:	Symplectic Sheffield
Date Deposited:	19 Jul 2017 09:00
Last Modified:	19 Jul 2017 09:03
Published Version:	https://doi.org/10.1186/s13104-017-2528-6
Status:	Published
Publisher:	BioMed Central
Refereed:	Yes
Identification Number:	https://doi.org/10.1186/s13104-017-2528-6
Related URLs:	Author

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The characterisation of microsatellite markers reveals tetraploidy in the Greater Water Parsnip, Sium latifolium (Apiaceae).

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