Barnard, A., Posocco, P., Fermeglia, M. et al. (4 more authors) (2014) Double-degradable responsive self-assembled multivalent arrays-temporary nanoscale recognition between dendrons and DNA. Organic and Biomolecular Chemistry. pp. 446-455. ISSN 1477-0520
Abstract
This article reports self-assembling dendrons which bind DNA in a multivalent manner. The molecular design directly impacts on self-assembly which subsequently controls the way these multivalent nanostructures bind DNA-this can be simulated by multiscale modelling. Incorporation of an S-S linkage between the multivalent hydrophilic dendron and the hydrophobic units responsible for self-assembly allows these structures to undergo triggered reductive cleavage, with dithiothreitol (DTT) inducing controlled breakdown, enabling the release of bound DNA. As such, the high-affinity self-assembled multivalent binding is temporary. Furthermore, because the multivalent dendrons are constructed from esters, a second slow degradation step causes further breakdown of these structures. This two-step double-degradation mechanism converts a large self-assembling unit with high affinity for DNA into small units with no measurable binding affinity-demonstrating the advantage of self-assembled multivalency (SAMul) in achieving highly responsive nanoscale binding of biological targets.
Metadata
Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Royal Society of Chemistry 2014. This is an author produced version of a paper accepted for publication in Organic & Biomolecular Chemistry. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 10 Dec 2015 14:11 |
Last Modified: | 06 Dec 2023 10:58 |
Published Version: | https://doi.org/10.1039/c3ob42202j |
Status: | Published |
Refereed: | Yes |
Identification Number: | https://doi.org/10.1039/c3ob42202j |
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