Effect of Different Carbon Concentration on Yield of Extracellular Polymeric Substances (EPS) Produced by Pseudomonas Putida ATCC 11172

The extracellular polymeric substances (EPS) extracted from Pseudomonas putida ATCC 11172 at different carbon concentration, where Luria broth used as medium growth with and without addition of 0.5% w/v (LBG 0.5%) and 1.0% w/v (LBG 1%) glucose. The Bound EPS were extracted by ethylene diamine tetra acetic acid (EDTA) methods and precipitated by cold ethanol 3:1; while free EPS extracted only by precipitate by cold ethanol 3:1. The study shows the clear effect of carbon concentration on the production of EPS. The amount of free EPS found 120.39±2.20, 111.16±1.75 and 100.38±2.0 mg g-1 dry cell for LB, LBG 0.5 and LBG 1 respectively. Where the yield of bound EPS shows fewer amounts from free EPS, the amount of bound EPS was 29.22±1.40, 21.18±0.90 and 17.10±1.10 mg g-1 dry cell for LB, LBG 0.5 and LBG 1 respectively.