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Pettit, L.J., Browne, H.P., Yu, L. et al. (10 more authors) (2014) Functional genomics reveals that Clostridium difficile Spo0A coordinates sporulation, virulence and metabolism. BMC Genomics, 15. 160. 160 - 160. ISSN 0094-8276
Abstract
Background Clostridium difficile is an anaerobic, Gram-positive bacterium that can reside as a commensal within the intestinal microbiota of healthy individuals or cause life-threatening antibiotic-associated diarrhea in immunocompromised hosts. C. difficile can also form highly resistant spores that are excreted facilitating host-to-host transmission. The C. difficile spo0A gene encodes a highly conserved transcriptional regulator of sporulation that is required for relapsing disease and transmission in mice.
Results Here we describe a genome-wide approach using a combined transcriptomic and proteomic analysis to identify Spo0A regulated genes. Our results validate Spo0A as a positive regulator of putative and novel sporulation genes as well as components of the mature spore proteome. We also show that Spo0A regulates a number of virulence-associated factors such as flagella and metabolic pathways including glucose fermentation leading to butyrate production.
Conclusions The C. difficile spo0A gene is a global transcriptional regulator that controls diverse sporulation, virulence and metabolic phenotypes coordinating pathogen adaptation to a wide range of host interactions. Additionally, the rich breadth of functional data allowed us to significantly update the annotation of the C. difficile 630 reference genome which will facilitate basic and applied research on this emerging pathogen.
Metadata
Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2014 Pettit et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
Keywords: | Clostridium difficile; Spo0A; RNAseq; Proteomics; Sporulation; Virulence; Metabolism; Butyrate; Transmission; Genome annotation |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 02 Mar 2015 10:22 |
Last Modified: | 23 Mar 2018 22:10 |
Published Version: | http://dx.doi.org/10.1186/1471-2164-15-160 |
Status: | Published |
Publisher: | BioMed Central |
Refereed: | Yes |
Identification Number: | https://doi.org/10.1186/1471-2164-15-160 |
Available Versions of this Item
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Functional genomics reveals that Clostridium difficile Spo0A coordinates sporulation, virulence and metabolism. (deposited 16 Apr 2014 13:55)
- Functional genomics reveals that Clostridium difficile Spo0A coordinates sporulation, virulence and metabolism. (deposited 02 Mar 2015 10:22) [Currently Displayed]
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Filename: 1471-2164-15-160-s1.pdf
Description: Identification of differentially expressed genes in C. difficile 630∆erm spo0A mutant by transcriptional profiling.
Filename: 1471-2164-15-160-s2.xlsx
Description: Table summarizing genes differentially expressed in a C. difficile spo0A mutant relative to the parental strain based on transcriptomics and proteomics.
Filename: 1471-2164-15-160-s3.docx
Description: Table summarizing gene regulation genes that are controlled by C. difficile Spo0A.