A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

Abstract

Background: Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. Results: We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. Conclusion: pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.

Metadata

Authors/Creators:

Fayed, Bahgat
Younger, Ellen
Taylor, Gabrielle
Smith, Maggie https://orcid.org/0000-0002-4150-0496

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Keywords:

Streptomyces, Cloning, Integration vector, Serine integrase, Bacteriophage, SV1

Dates: