Trikic, M.Z., Monk, P., Roehl, H. et al. (1 more author) (2011) Regulation of Zebrafish Hatching by Tetraspanin cd63. PLoS One, 6 (5). e19683. ISSN 1932-6203
Abstract
Tetraspanins cause the clustering of membrane proteins into a level of organisation essential for cellular function. Given the importance and complicated nature of this mechanism, we attempted a novel approach to identify the function of a single component in a biologically relevant context. A morpholino knockdown strategy was used to investigate the role of cd63, a membrane protein associated with intracellular transport and a melanoma marker, in embryonic zebrafish. By using three separate morpholinos targeting cd63, we were able to identify a specific phenotype. Strikingly, morphant fish failed to hatch due to the lack of secreted proteolytic enzymes required for chorion-softening. The morphology of the hatching gland at both the cellular and intracellular levels was disorganised, suggesting a role for cd63 in the functioning of this organ. This work identifies a specific role for cd63 in the zebrafish embryo and provides evidence for the suitability of zebrafish as a model system for the investigation of tetraspanin enriched microdomains.
Metadata
Authors/Creators: |
|
---|---|
Copyright, Publisher and Additional Information: | © 2011 Trikić et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Keywords: | CATHEPSIN-L; SECRETORY LYSOSOMES; GENE-EXPRESSION; DENDRITIC CELLS; PROTEIN CD63; WILD-TYPE; DIFFERENTIATION; MEMBRANE; VESICLES; BIOSYNTHESIS |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection and Immunity (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 10 Nov 2014 13:38 |
Last Modified: | 10 Nov 2014 13:44 |
Published Version: | http://dx.doi.org/10.1371/journal.pone.0019683 |
Status: | Published |
Publisher: | Public Library of Science |
Refereed: | Yes |
Identification Number: | https://doi.org/10.1371/journal.pone.0019683 |