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The two pore channel TPK1 gene encodes the vacuolar K+ conductance and plays a role in K+ homeostasis

Gobert, A., Isayenkov, S., Voelker, C., Czempinski, K. and Maathuis, F.J.M. (2007) The two pore channel TPK1 gene encodes the vacuolar K+ conductance and plays a role in K+ homeostasis. Proceedings of the National Academy of Sciences of the United States of America, 104 (25). pp. 10726-10731. ISSN 0027-8424

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Abstract

The Arabidopsis thaliana genome contains five genes that encode two pore K+ (TPK) channels. The most abundantly expressed isoform of this family, TPK1, is expressed at the tonoplast where it mediates K+-selective currents between cytoplasmic and vacuolar compartments. TPK1 open probability depends on both cytoplasmic Ca2+ and cytoplasmic pH but not on the tonoplast membrane voltage. The channel shows intrinsic rectification and can be blocked by Ba2+, tetraethylammonium, and quinine. TPK1 current was found in all shoot cell types and shows all of the hallmarks of the previously described vacuolar K (VK) tonoplast channel characterized in guard cells. Characterization of TPK1 loss-of-function mutants and TPK1-overexpressing plants shows that TPK1 has a role in intracellular K+ homeostasis affecting seedling growth at high and low ambient K+ levels. In stomata, TPK1 function is consistent with vacuolar K+ release, and removal of this channel leads to slower stomatal closure kinetics. During germination, TPK1 contributes to the radicle development through vacuolar K+ deposition to provide expansion growth or in the redistribution of essential minerals.

Item Type: Article
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 17 Feb 2009 11:07
Last Modified: 17 Feb 2009 11:07
Published Version: http://dx.doi.org/10.1073/pnas.0702595104
Status: Published
Publisher: National Academy of Sciences; 1999
Identification Number: 10.1073/pnas.0702595104
URI: http://eprints.whiterose.ac.uk/id/eprint/7397

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