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Viral cyclin-cyclin-dependent kinase 6 complexes initiate nuclear DNA replication

Laman, H., Coverley, D., Krude, T., Laskey, R. and Jones, N. (2001) Viral cyclin-cyclin-dependent kinase 6 complexes initiate nuclear DNA replication. Molecular and Cellular Biology, 21 (2). pp. 624-635. ISSN 0270-7306

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The cyclins encoded by Kaposi sarcoma-associated herpesvirus and herpesvirus saimiri are homologs of human D-type cyclins. However, when complexed to cdk6, they have several activities that distinguish them from D-type cyclin-cdk6 complexes, including resistance to cyclin-dependent kinase inhibitors and an enhanced substrate range. We find that viral cyclins interact with and phosphorylate proteins involved in replication initiation. Using mammalian in vitro replication systems, we show that viral cyclin-cdk6 complexes can directly trigger the initiation of DNA synthesis in isolated late-G1-phase nuclei. Viral cyclin-cdk6 complexes share this capacity with cyclin A-cdk2, demonstrating that in addition to functioning as G1-phase cyclin-cdk complexes, they function as S-phase cyclin-cdk complexes.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 26 Mar 2009 09:51
Last Modified: 26 Mar 2009 09:51
Published Version: http://dx.doi.org/10.1128/MCB.21.2.624-635.2001
Status: Published
Publisher: American Society for Microbiology
Identification Number: 10.1128/MCB.21.2.624-635.2001
URI: http://eprints.whiterose.ac.uk/id/eprint/7022

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