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X-ray diffraction from DNA fibres under tension

Greenall, R.J., Nave, C. and Fuller, W. (2001) X-ray diffraction from DNA fibres under tension. Journal of Molecular Biology, 305 (4). pp. 669-672. ISSN 0022-2836

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Abstract

When DNA fibres are stretched during drying, the polymer undergoes a conformational transition. We present quantitative results from X-ray diffraction studies on such fibres held at various ambient relative humidities. These indicate that the molecules are arranged in arrays which are crystalline in projection down the fibre axis. The packing can be explained in terms of a hexagonal cell with a lattice parameter, a, of 13 Å which varies with humidity. The patterns contain meridional intensities at 1/3.4 Å−1 and 1/6.5 Å−1, a strong off-meridional intensity at Z=1/5.6 Å−1 and diffuse scatter at Z=1/28 Å−1.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Physics (York)
Depositing User: York RAE Import
Date Deposited: 17 Apr 2009 15:07
Last Modified: 17 Apr 2009 15:07
Published Version: http://dx.doi.org/10.1006/jmbi.2000.4325
Status: Published
Publisher: Elsevier Science B.V.
Identification Number: 10.1006/jmbi.2000.4325
URI: http://eprints.whiterose.ac.uk/id/eprint/6751

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