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CD40/CD154 interactions are required for the optimal maturation of skin-derived APCs and the induction of Helminth-specific IFN-? but not IL-4

Hewitson, J.P., Jenkins, G.R., Hamblin, P.A. and Mountford, A.P. (2006) CD40/CD154 interactions are required for the optimal maturation of skin-derived APCs and the induction of Helminth-specific IFN-? but not IL-4. Journal of Immunology, 177 (5). pp. 3209-3217. ISSN 0022-1767

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Abstract

The mechanisms through which Schistosoma mansoni larvae induce Th1 rather than Th2 immune responses are not well understood. In this study, using CD154–/– mice exposed to radiation-attenuated S. mansoni larvae, we demonstrate roles for CD154/CD40 in the activation of skin-derived APCs and the development of Th1 cells in the skin-draining lymph nodes (sdLN). The presence of CD154 was important for optimal IL-12p40 and essential for Ag-specific IFN-, but CD154 expression by wild-type CD4– cells was insufficient to rescue recall responses of CD4+ cells from CD154–/– mice. This defect is probably due to impaired CD40-dependent IL-12 production in vivo, because administration of anti-CD40 Ab, or rIL-12, restored IFN- production by sdLN cells from CD154–/– mice. CD154 ligation of CD40 was not required for the migration of skin-derived APCs, but did have a limited role in their maturation (increased MHC II and CD86). Unexpectedly, although CD4 cells from CD154–/– mice were deficient in their ability to produce IFN-, they produced significant amounts of IL-4 and IL-5 in the presence of skin-derived APCs from wild-type and CD154–/– mice. Thus, in contrast to IFN-, the production of Th2-associated cytokines is (in this model) independent of CD154. We conclude that whereas the priming of Th1 responses soon after exposure to schistosome larvae is completely CD40/CD154 dependent, IL-4, IL-5, and IL-13 are independent of CD154, suggesting a dichotomy in the specific mechanisms that induce these cytokines by CD4+ cells in the sdLN.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 22 Apr 2009 10:58
Last Modified: 22 Apr 2009 10:58
Published Version: http://www.jimmunol.org/cgi/content/abstract/177/5...
Status: Published
Publisher: American Association of Immunologists
URI: http://eprints.whiterose.ac.uk/id/eprint/6718

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