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Bcl-2 constitutively suppresses p53-dependent apoptosis in colorectal cancer cells

Jiang, M. and Milner, J. (2003) Bcl-2 constitutively suppresses p53-dependent apoptosis in colorectal cancer cells. Genes and Development, 17 (7). pp. 832-837. ISSN 0890-9369

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To dissect apoptotic genes governing the survival of colorectal carcinoma cells, we employed RNAi to silence Bcl-2 and Bcl-xLin isogenic clones of p53+/+ and p53−/− cells, and of Bax+/− and Bax−/− cells. We identify a novel proapoptotic function of p53 that does not require activation by genotoxic agents and that appears to be constitutively suppressed by Bcl-2. Silencing of Bcl-2 induced massive p53-dependent apoptosis. The “Bcl-2/p53 axis” requires Bax and caspase 2 as essential apoptotic mediators. This newly discovered Bcl-2/p53 functional interface represents a key regulator of apoptosis which can be activated by targeting Bcl-2 in colorectal carcinoma cells.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 27 May 2009 13:41
Last Modified: 27 May 2009 13:41
Published Version: http://dx.doi.org/10.1101/gad.252603
Status: Published
Publisher: Elsevier
Identification Number: 10.1101/gad.252603
URI: http://eprints.whiterose.ac.uk/id/eprint/6101

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