White Rose University Consortium logo
University of Leeds logo University of Sheffield logo York University logo

Estimating hydration changes upon biomolecular reactions from osmotic stress, high pressure, and preferential hydration experiments

Shimizu, S (2004) Estimating hydration changes upon biomolecular reactions from osmotic stress, high pressure, and preferential hydration experiments. Proceedings of the National Academy of Sciences, USA. pp. 1195-1199. ISSN 0027-8424

Full text available as:
[img]
Preview
Text (shimizus1.pdf)
shimizus1.pdf

Download (234Kb)

Abstract

How do we estimate, from thermodynamic measurements, the number of water molecules adsorbed or released from biomolecules as a result of a biochemical process such as binding and allosteric effects? Volumetric and osmotic stress analyses are established methods for estimating water numbers; however, these techniques often yield conflicting results. In contrast, Kirkwood-Buff theory offers a novel way to calculate excess hydration number from volumetric data, provides a quantitative condition to gauge the accuracy of osmotic stress analysis, and clarifies the relationship between osmotic and volumetric analyses. I have applied Kirkwood-Buff theory to calculate water numbers for two processes: (i) the allosteric transition of hemoglobin and (h) the binding of camphor to cytochrome P450. I show that osmotic stress analysis may overestimate hydration number changes for these processes.

Item Type: Article
Copyright, Publisher and Additional Information: © 2004 by The National Academy of Sciences of the USA
Keywords: water, partial molar volume, preferential interaction, hemoglobin, cytochrome P450, PARTIAL MOLAR VOLUME, PROTEIN HYDRATION, HYDROPHOBIC SOLUTES, BINDING, WATER, HEMOGLOBIN, THERMODYNAMICS, SOLVATION, SOLVENT, COMPRESSIBILITY
Academic Units: The University of York > Chemistry (York)
Depositing User: Repository Officer
Date Deposited: 01 Jul 2005
Last Modified: 17 Oct 2013 14:27
Published Version: http://dx.doi.org/10.1073/pnas.0305836101
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/543

Actions (login required)

View Item View Item