Shimizu, S (2004) Estimating hydration changes upon biomolecular reactions from osmotic stress, high pressure, and preferential hydration experiments. Proceedings of the National Academy of Sciences, USA. pp. 1195-1199. ISSN 0027-8424Full text available as:
How do we estimate, from thermodynamic measurements, the number of water molecules adsorbed or released from biomolecules as a result of a biochemical process such as binding and allosteric effects? Volumetric and osmotic stress analyses are established methods for estimating water numbers; however, these techniques often yield conflicting results. In contrast, Kirkwood-Buff theory offers a novel way to calculate excess hydration number from volumetric data, provides a quantitative condition to gauge the accuracy of osmotic stress analysis, and clarifies the relationship between osmotic and volumetric analyses. I have applied Kirkwood-Buff theory to calculate water numbers for two processes: (i) the allosteric transition of hemoglobin and (h) the binding of camphor to cytochrome P450. I show that osmotic stress analysis may overestimate hydration number changes for these processes.
|Copyright, Publisher and Additional Information:||© 2004 by The National Academy of Sciences of the USA|
|Keywords:||water, partial molar volume, preferential interaction, hemoglobin, cytochrome P450, PARTIAL MOLAR VOLUME, PROTEIN HYDRATION, HYDROPHOBIC SOLUTES, BINDING, WATER, HEMOGLOBIN, THERMODYNAMICS, SOLVATION, SOLVENT, COMPRESSIBILITY|
|Academic Units:||The University of York > Chemistry (York)|
|Depositing User:||Repository Officer|
|Date Deposited:||01 Jul 2005|
|Last Modified:||17 Oct 2013 14:27|