Kobayashi, M., Kidd, D., Hutson, E., Grafton, J., McNulty, S. and Rumsby, M. (2001) Protein kinase C activation by 12-0-tetradecanoylphorbol 13-acetate in CG-4 line oligodendrocytes stimulates turnover of choline and ethanolamine phospholipids by phospholipase D and induces rapid process contraction. Journal Of Neurochemistry, 76 (2). pp. 361-371. ISSN 0022-3042Full text not available from this repository.
Treatment of [3H]-choline- or [14C]-ethanolamine-labelled undifferentiated bipolar and differentiated multipolar CG-4 line oligodendrocytes with 12–0-tetradecanoylphorbol 13-acetate (TPA) to activate protein kinase C stimulated the release of choline or ethanolamine metabolites to the medium over controls. Ro31–8220, a PKC inhibitor, reduced TPA-stimulated release of choline- and ethanolamine-metabolites to basal levels. TPA treatment of both bipolar and multipolar cells caused rapid contraction of processes leaving rounded up cells: this effect was blocked by Ro31–8220. After 12–15 h exposure to TPA, bipolar undifferentiated CG-4 line cells extended short processes again and the cells became multipolar. Nocodozole, an agent which disrupts microtubules and caused CG-4 line cells to round up, caused increased choline or ethanolamine-metabolite release to the medium over basal levels suggesting that some release during TPA-treatment might occur due to process fragmentation. However, the transphosphatidylation reaction confirmed that phospholipase D was active in these cells. Exposure of bipolar undifferentiated CG-4 line cells to TPA resulted in down-regulatation of PKC-α and PKC-βΙΙ which could not be detected by Western blotting after a few hours; PKC-ε was down-regulated much more slowly but PKCs δ, ζ and ι were not influenced by 48 h exposure of cells to TPA. Formation of phosphatidylethanol in the transphosphatidylation reaction was markedly reduced in TPA down-regulated cells indicating a role for PKCs α and βΙΙ in phospholipase D activation in CG-4 line oligodendrocytes.
|Copyright, Publisher and Additional Information:||Open access copy available from the journal web site.|
|Keywords:||CG-4 line oligodendrocytes, protein kinase C subspecies alpha and beta(II), phospholipase D, choline and ethanolamine phospholipid hydolysis, transphosphatidylation, process contraction|
|Institution:||The University of York|
|Academic Units:||The University of York > Biology (York)|
|Depositing User:||Open Access From Journal|
|Date Deposited:||22 Dec 2008 15:05|
|Last Modified:||22 Dec 2008 15:05|