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Lysophosphatidic acid induces process retraction in CG-4 line oligodendrocytes and oligodendrocyte precursor cells but not in differentiated oligodendrocytes

Dawson, J., Hotchin, N., Lax, S. and Rumsby, M. (2003) Lysophosphatidic acid induces process retraction in CG-4 line oligodendrocytes and oligodendrocyte precursor cells but not in differentiated oligodendrocytes. Journal of Neurochemistry, 87 (4). pp. 947-957. ISSN 0022-3042

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Abstract

Lysophosphatidic acid is a growth factor-like signalling phospholipid. We demonstrate here that lysophosphatidic acid induces process retraction in central glia-4 cells and oligodendrocyte precursors. This lysophosphatidic acid effect is rapid and concentration-dependent and results in cell rounding. It is inhibited by pre-treatment of cells with C3 exoenzyme, a specific inhibitor of Rho, or with Y-27632, a specific inhibitor of ROCK, a downstream kinase of Rho. Processes of differentiated central glia-4 oligodendrocytes were insensitive to lysophosphatidic acid treatment but cell bodies became phase dark, indicating cell spreading on the poly-l-lysine substratum. RT-PCR and Western blot analyses indicate that oligodendrocyte precursors and mature oligodendrocytes express mRNA and protein for LPA1, one of several LPA receptors. Thus lysophosphatidic acid may be signalling to Rho and stimulating actomyosin contraction in precursor oligodendrocytes by this family of receptors. The results show that lysophosphatidic acid signalling pathways influence retraction of processes in oligodendrocyte precursors but that this effect changes as oligodendrocytes differentiate.

Item Type: Article
Copyright, Publisher and Additional Information: Open access copy available from the journal web site.
Keywords: CG-4 cells, LPA1 receptor, lysophosphatidic acid, oligodendrocytes, process retraction, S1P
Academic Units: The University of York > Biology (York)
Depositing User: Open Access From Journal
Date Deposited: 22 Dec 2008 19:42
Last Modified: 22 Dec 2008 19:42
Published Version: http://dx.doi.org/10.1046/j.1471-4159.2003.02056.x
Status: Published
Publisher: Wiley-Blackwell
Refereed: Yes
Identification Number: 10.1046/j.1471-4159.2003.02056.x
URI: http://eprints.whiterose.ac.uk/id/eprint/5090

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