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Persistence of inert macromolecules (imposil)in the rat mesangium and glomerular funtional disturbance

Goode, NP, Davison, AM, Gowland, G, Aparacio, SR and Shires, M (1984) Persistence of inert macromolecules (imposil)in the rat mesangium and glomerular funtional disturbance. Journal of Pathology, 144 (3). 179 - 187 . ISSN 0022-3417


Imposil iron-dextran is an inert tracer that has been used to study mesangial uptake and clearance of macromolecular material from the glomerular circulation. Such a tracer may be a useful marker of altered mesangial function in animals with some forms of glomerulonephritis. We have studied mesangial handling of intravenously injected Imposil (50 mg/100 g body weight) in normal rats by light, immunofluorescence and electron microscopy for up to 3 months. Mesangial cell uptake was maximal at 48-54 h. Extrusion and drainage of tracer to the vascular pole and distal tubule was evident at 3 days but iron was still present in mesangial cells at 3 months. Possible functional renal impairment resulting from persistent mesangially sequestered tracer was examined by measuring daily urine protein and iron excretion. A possible relationship between failure of mesangial cells to eliminate inert tracer and increasing glomerular permeability is demonstrated, suggesting that Imposil and similar inert macromolecules cannot be used for long-term studies of mesangial function.

Item Type: Article
Institution: The University of Leeds
Academic Units: The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds)
Depositing User: Symplectic Publications
Date Deposited: 25 Apr 2012 09:43
Last Modified: 15 Sep 2014 03:40
Published Version: http://dx.doi.org/10.1002/path.1711440305
Status: Published
Publisher: John Wiley & Sons
Identification Number: 10.1002/path.1711440305
URI: http://eprints.whiterose.ac.uk/id/eprint/43875

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