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Preparation and use of the poly-L-lysine-gold probe: a differential marker of glomerular anionic sites.

Goode, NP, Shires, M and Davison, AM (1992) Preparation and use of the poly-L-lysine-gold probe: a differential marker of glomerular anionic sites. Histochemistry and Cell Biology, 98 (1). 67 - 72 . ISSN 0301-5564

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Abstract

The conditions required for the production of a polylysine-coated gold (PL-G) complex, which shows optimal sensitivity for the demonstration of tissue anionic sites, expressed under different conditions of pH have been investigated. Problems encountered with this complex have been compared with those found with other methods of conjugation of polylysine to colloidal gold. The performance of a bovine serum albumin (BSA)-stabilized PL-G complex was examined against other PL-G conjugates, including complexes that are commercially available, for the detection of heterogeneous glomerular anionic site populations, expressed at pH 2.5 and pH 7.0.

Item Type: Article
Keywords: anions, drug stability, gold, immunohistochemistry, kidney glomerulus, polylysine, serum albumin, bovine, staining and labeling
Institution: The University of Leeds
Academic Units: The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds)
Depositing User: Symplectic Publications
Date Deposited: 20 Apr 2012 08:31
Last Modified: 08 Feb 2013 17:37
Published Version: http://http/dx.doi.org/10.1007/BF00716938
Status: Published
Publisher: Springer Verlag
Identification Number: 10.1007/BF00716938
URI: http://eprints.whiterose.ac.uk/id/eprint/43868

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