Goode, NP, Shires, M and Davison, AM (1992) Preparation and use of the poly-L-lysine-gold probe: a differential marker of glomerular anionic sites. Histochemistry and Cell Biology, 98 (1). 67 - 72 . ISSN 0301-5564
The conditions required for the production of a polylysine-coated gold (PL-G) complex, which shows optimal sensitivity for the demonstration of tissue anionic sites, expressed under different conditions of pH have been investigated. Problems encountered with this complex have been compared with those found with other methods of conjugation of polylysine to colloidal gold. The performance of a bovine serum albumin (BSA)-stabilized PL-G complex was examined against other PL-G conjugates, including complexes that are commercially available, for the detection of heterogeneous glomerular anionic site populations, expressed at pH 2.5 and pH 7.0.
|Keywords:||anions, drug stability, gold, immunohistochemistry, kidney glomerulus, polylysine, serum albumin, bovine, staining and labeling|
|Institution:||The University of Leeds|
|Academic Units:||The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds)|
|Depositing User:||Symplectic Publications|
|Date Deposited:||20 Apr 2012 08:31|
|Last Modified:||15 Sep 2014 03:40|