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Post-embedding double-labeling of antigen-retrieved ultrathin sections using a silver enhancement-controlled sequential immunogold (SECSI) technique.

Goode, NP, Shires, M, Crellin, DM, Khan, TN and Mooney, AF (2004) Post-embedding double-labeling of antigen-retrieved ultrathin sections using a silver enhancement-controlled sequential immunogold (SECSI) technique. Journal of Histochemistry and Cytochemistry, 52 (1). 141 - 144 . ISSN 0022-1554

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Abstract

In electron microscopy, the post-embedding immunogold technique provides a high degree of resolution and the possibility of quantitation owing to the intrinsic characteristics of the colloidal gold marker. Application of this technique to the subcellular localization of multiple antigens by differential labeling using gold markers of different sizes, or to double labeling using the same primary antibody isotype with serial silver enhancement, has been reported. We have incorporated this double labeling technique into a modified procedure that produces excellent labeling and ultrastructural preservation, even after exposure of ultrathin sections large enough to cover a 300- micro m-diameter single-hole grid to hot antigen retrieval solutions and prolonged labeling protocols.

Item Type: Article
Keywords: Actinin, Antigens, CD29, Cryoultramicrotomy, Epitopes, Humans, Immunohistochemistry, Kidney, Microfilament Proteins, Silver Staining
Academic Units: The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds)
Depositing User: Symplectic Publications
Date Deposited: 16 Apr 2012 10:46
Last Modified: 08 Feb 2013 17:37
Published Version: http://dx.doi.org/10.1177/002215540405200114
Status: Published
Publisher: Sage Publications
Identification Number: 10.1177/002215540405200114
URI: http://eprints.whiterose.ac.uk/id/eprint/43855

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