Goode, NP, Shires, M, Crellin, DM, Khan, TN and Mooney, AF (2004) Post-embedding double-labeling of antigen-retrieved ultrathin sections using a silver enhancement-controlled sequential immunogold (SECSI) technique. Journal of Histochemistry and Cytochemistry, 52 (1). 141 - 144 . ISSN 0022-1554Full text available as:
In electron microscopy, the post-embedding immunogold technique provides a high degree of resolution and the possibility of quantitation owing to the intrinsic characteristics of the colloidal gold marker. Application of this technique to the subcellular localization of multiple antigens by differential labeling using gold markers of different sizes, or to double labeling using the same primary antibody isotype with serial silver enhancement, has been reported. We have incorporated this double labeling technique into a modified procedure that produces excellent labeling and ultrastructural preservation, even after exposure of ultrathin sections large enough to cover a 300- micro m-diameter single-hole grid to hot antigen retrieval solutions and prolonged labeling protocols.
|Keywords:||Actinin, Antigens, CD29, Cryoultramicrotomy, Epitopes, Humans, Immunohistochemistry, Kidney, Microfilament Proteins, Silver Staining|
|Institution:||The University of Leeds|
|Academic Units:||The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds)|
|Depositing User:||Symplectic Publications|
|Date Deposited:||16 Apr 2012 10:46|
|Last Modified:||15 Sep 2014 03:40|