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Biotransformation of explosives by the old yellow enzyme family of flavoproteins

Williams, R.E., Rathbone, D.A., Bruce, N.C. (orcid.org/0000-0003-0398-2997) and Scrutton, N.S. (2004) Biotransformation of explosives by the old yellow enzyme family of flavoproteins. Applied and Environmental Microbiology. pp. 3566-3574. ISSN 0099-2240

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Several independent studies of bacterial degradation of nitrate ester explosives have demonstrated the involvement of flavin-dependent oxidoreductases related to the old yellow enzyme (OYE) of yeast. Some of these enzymes also transform the nitroaromatic explosive 2,4,6-trinitrotoluene (TNT). In this work, catalytic capabilities of five members of the OYE family were compared, with a view to correlating structure and function. The activity profiles of the five enzymes differed substantially; no one compound proved to be a good substrate for all five enzymes. TNT is reduced, albeit slowly, by all five enzymes. The nature of the transformation products differed, with three of the five enzymes yielding products indicative of reduction of the aromatic ring. Our findings suggest two distinct pathways of TNT transformation, with the initial reduction of TNT being the key point of difference between the enzymes. Characterization of an active site mutant of one of the enzymes suggests a structural basis for this difference.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2004 American Society for Microbiology
Institution: The University of York
Academic Units: The University of York > Biology (York) > Centre for Novel Agricultural Products (CNAP) (York)
Depositing User: Sherpa Assistant
Date Deposited: 01 Apr 2005
Last Modified: 07 Aug 2016 00:04
Published Version: http://dx.doi.org/10.1128/AEM.70.6.3566â¿¿3574.200...
Status: Published
Refereed: Yes
URI: http://eprints.whiterose.ac.uk/id/eprint/374

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