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Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a charge-coupled device camera

Lancaster, M., Goodall, D.M., Bergstrom, E.T., McCrossen, S. and Myers, P. (2008) Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a charge-coupled device camera. Journal of Chromatography A. pp. 219-225. ISSN 0021-9673

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Abstract

This paper presents the first study of the UV imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging of spots of benzophenone during and after development was carried out using a charge-coupled device camera. Limits of detection were found to be 5 ng on a wetted plate and 3 ng for a dry plate and the relationship between peak area and sample loading was found to be linear in the low nanograrn range over an order of magnitude for both wet and dry modes with r(2) values > 0.99. It was found that UV measurements on wet glass-backed plates suffer from low sensitivity; however, the use of aluminium-backed plates gave increased sensitivity. The apparent absorption coefficient (epsilon(app)) of 10AU m(2) g(-1) at 254 nm is consistent with reflection of the light from the aluminium surface with a double pass through the sorbent layer, and suggests that use of aluminium-backed plates should enable monitoring of separations by UV absorbance during TLC development. (c) 2008 Elsevier B.V. All rights reserved.

Item Type: Article
Copyright, Publisher and Additional Information: © 2008 Elsevier B.V. This is an author produced version of a paper published in Journal of Chromatography A. Uploaded in accordance with the publisher's self-archiving policy.
Keywords: thin-layer chromatography, videodensitometry, CCD detection, VIDEODENSITOMETRIC TLC, CCD IMAGERS, TABLETS, DISPERSION, SOLVENTS, HPTLC, Analytical Chemistry
Institution: The University of York
Academic Units: The University of York > Chemistry (York)
Depositing User: Sherpa Assistant
Date Deposited: 04 Apr 2008 10:45
Last Modified: 14 Oct 2014 09:24
Published Version: http://dx.doi.org/10.1016/j.chroma.2008.01.016
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/3722

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