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Isolation of polymorphic microsatellites in the stemless thistle (Cirsium acaule) and their utility in other Cirsium species

Jump, A.S., Dawson, D.A., James, C.M., Woodward, F.I. and Burke, T. (2002) Isolation of polymorphic microsatellites in the stemless thistle (Cirsium acaule) and their utility in other Cirsium species. Molecular Ecology Notes, 2 (4). pp. 589-592. ISSN 1471-8278


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The genus Cirsium includes species with both widespread and restricted geographical distributions, several of which are serious weeds. Nine polymorphic microsatellite loci were isolated from the stemless thistle Cirsium acaule. Eight were polymorphic in C. acaule, six in C. arvense and seven in C. heterophyllum. One locus monomorphic in C. acaule showed polymorphism in C. heterophyllum. The mean number of alleles per locus was 4.1 in C. acaule, 6.2 in C. arvense and 2.9 in C. heterophyllum. These nine loci were also amplified in C. eriophorum and C. vulgare, suggesting that these markers may be of use throughout the genus.

Item Type: Article
Copyright, Publisher and Additional Information: © 2002 Blackwell Science Ltd. This is an electronic version of an article published in Molecular Ecology Notes: complete citation information for the final version of the paper, as published in the print edition of Molecular Ecology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwellpublishing.com/journal.asp?ref=1471-8278 or http://www.blackwell-synergy.com/
Keywords: Asteraceae, Cirsium, cross-species amplification, microsatellite, thistle, weed
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield)
Depositing User: Sherpa Assistant
Date Deposited: 31 Mar 2005
Last Modified: 05 Jun 2014 07:54
Published Version: http://www.blackwell-synergy.com/
Status: Published
Refereed: Yes
Identification Number: 10.1046/j.1471-8286.2002.00329.x
URI: http://eprints.whiterose.ac.uk/id/eprint/365

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