Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo

Stepek, G., Lowe, A.E., Buttle, D.J., Duce, I.R. and Behnke, J.M. (2007) Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo. Parasitology, 134 (1). pp. 103-112. ISSN 0031-1820

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Abstract

Cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect, in vitro, against the rodent gastrointestinal nematodes, H eligmosomoides polygyrus (which is found in the anterior small intestine) and Trichuris miti,is (which resides in the caecum). Proteinases in the crude latex of papaya also showed anthelmintic efficacy against both nematodes in vivo. In this paper, we describe the in vitro and in vivo effects of these plant extracts against the rodent nematode, Protospirua muricola, which is found in the stomach. As in earlier work, all the plant cysteine proteinases examined, with the exception of actinidain from the juice of kiwi fruit, caused rapid loss of motility and digestion of the cuticle, leading to death of the nematode in vitro. In vivo, in contrast to the efficacy against H. polygyrus and T. muris, papaya latex only showed efficacy against P. muricola adult female worms when the stomach acidity had been neutralized prior to administration of papaya latex. Therefore, collectively, our studies have demonstrated that, with the appropriate formulation, plant cysteine proteinases have efficacy against nematodes residing throughout the rodent gastrointestinal tract.

Item Type:	Article
Copyright, Publisher and Additional Information:	© 2007 Cambridge University Press. This paper will be made live in Jan 2008.
Keywords:	Protospirura muricola, plant cysteine proteinases, papaya, pineapple, fig, in vitro, in vivo, antacid, anthelmintic, gastrointestinal nematodes
Academic Units:	The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine (Sheffield) > Division of Genomic Medicine (Sheffield) > Section of Functional Genomics (Sheffield)
Depositing User:	Repository Officer
Date Deposited:	08 Aug 2007 14:08
Last Modified:	08 Feb 2013 16:54
Published Version:	http://dx.doi.org/10.1017/S0031182006001302
Status:	Published
Publisher:	Cambridge University Press
Refereed:	Yes
Identification Number:	10.1017/S0031182006001302
URI:	http://eprints.whiterose.ac.uk/id/eprint/2603

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