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Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia

Gaunt, M.W., Rigottier-Gois, L., Lloyd-Macgilp, S.A., Young, J.P.W. and Turner, S.L. (2001) Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. International Journal of Sytematic and Evolutionary Microbiology. pp. 2037-2048. ISSN 1466-5034

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Abstract

The current classification of the rhizobia (root-nodule symbionts) assigns them to six genera. It is strongly influenced by the small subunit (16S, SSU) rRNA molecular phylogeny, but such single-gene phylogenies may not reflect the evolution of the genome as a whole. To test this, parts of the atpD and recA genes have been sequenced for 25 type strains within the alpha -Proteobacteria, representing species in Rhizobium, Sinorhizobium, Mesorhizobium, Bradyrhizobium, Azorhizobium, Agrobacterium, Phyllobacterium, Mycoplana and Brevundimonas. The current genera Sinorhizobium and Mesorhizobium are well supported by these genes, each forming a distinct phylogenetic clade with unequivocal bootstrap support. There is good support for a Rhizobium clade that includes Agrobacterium tumefaciens, and the very close relationship between Agrobacterium rhizogenes and Rhizobium tropici is confirmed. There is evidence for recombination within the genera Mesorhizobium and Sinorhizobium, but the congruence of the phylogenies at higher levels indicates that the genera are genetically isolated. rRNA provides a reliable distinction between genera, but genetic relationships within a genus may be disturbed by recombination.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright © 2001 Society for General Microbiology
Keywords: Rhizobium, Sinorhizobium, Mesorhizobium, recA, atpD, FRAGMENT-LENGTH-POLYMORPHISM, ROOT-NODULE BACTERIA, CICER-ARIETINUM L, SP-NOV, SP. NOV., RHODOBACTER-SPHAEROIDES, HELICOBACTER-PYLORI, NUCLEOTIDE-SEQUENCE, MAXIMUM-LIKELIHOOD, GEN-NOV
Academic Units: The University of York > Biology (York)
Depositing User: Peter Young
Date Deposited: 29 Nov 2004
Last Modified: 17 Oct 2013 14:34
Status: Published
Refereed: Yes
Related URLs:
URI: http://eprints.whiterose.ac.uk/id/eprint/209

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