Hoh, Soon Wen orcid.org/0000-0003-1039-8000, Burnley, Tom and Cowtan, Kevin orcid.org/0000-0002-0189-1437 (2020) Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM. Acta crystallographica. Section D, Structural biology. pp. 531-541. ISSN 2059-7983
Abstract
This work focuses on the use of the existing protein-model-building software Buccaneer to provide structural interpretation of electron cryo-microscopy (cryo-EM) maps. Originally developed for application to X-ray crystallography, the necessary steps to optimise the usage of Buccaneer with cryo-EM maps are shown. This approach has been applied to the data sets of 208 cryo-EM maps with resolutions of better than 4 Å. The results obtained also show an evident improvement in the sequencing step when the initial reference map and model used for crystallographic cases are replaced by a cryo-EM reference. All other necessary changes to settings in Buccaneer are implemented in the model-building pipeline from within the CCP-EM interface (as of version 1.4.0).
Metadata
Authors/Creators: |
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Keywords: | Buccaneer, CCP-EM, Collaborative Computational Project for Electron cryo-Microscopy, Cryo-EM, Model building | ||||
Dates: |
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Institution: | The University of York | ||||
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) | ||||
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Depositing User: | Pure (York) | ||||
Date Deposited: | 12 Jun 2020 09:30 | ||||
Last Modified: | 04 Feb 2024 01:07 | ||||
Published Version: | https://doi.org/10.1107/S2059798320005513 | ||||
Status: | Published | ||||
Refereed: | Yes | ||||
Identification Number: | https://doi.org/10.1107/S2059798320005513 | ||||
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