Brabham, Robin, Keenan, Tessa, Husken, Annika et al. (5 more authors) (2020) Rapid sodium periodate cleavage of an unnatural amino acid enables unmasking of a highly reactive α-oxo aldehyde for protein bioconjugation. Organic and Biomolecular Chemistry. ISSN 1477-0520
Abstract
The α-oxo aldehyde is a highly reactive aldehyde for which many protein bioconjugation strategies exist. Here, we explore the genetic incorporation of a threonine-lysine dipeptide into proteins, harbouring a “masked” α-oxo aldehyde that is rapidly unveiled in four minutes. The reactive aldehyde could undergo site-specific protein modification by SPANC ligation
Metadata
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Institution: | The University of York | ||||
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) | ||||
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Depositing User: | Pure (York) | ||||
Date Deposited: | 20 May 2020 08:30 | ||||
Last Modified: | 06 Dec 2023 13:41 | ||||
Published Version: | https://doi.org/10.1039/d0ob00972e | ||||
Status: | Published online | ||||
Refereed: | Yes | ||||
Identification Number: | https://doi.org/10.1039/d0ob00972e |
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Filename: d0ob00972e.pdf
Description: Rapid sodium periodate cleavage of an unnatural amino acid enables unmasking of a highly reactive α-oxo aldehyde for protein bioconjugation
Licence: CC-BY 2.5