Auxin regulates SCFTIR1-dependent degradation of AUX/IAA proteins

Gray, W.M., Leyser, O., Estelle, M., Kepinski, S. and Rouse, D. (2001) Auxin regulates SCFTIR1-dependent degradation of AUX/IAA proteins. Nature. pp. 271-276. ISSN 0028-0836

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Abstract

The plant hormone auxin is central in many aspects of plant development. Previous studies have implicated the ubiquitin-ligase SCFTIR1 and the AUX/IAA proteins in auxin response. Dominant mutations in several AUX/IAA genes confer pleiotropic auxin-related phenotypes, whereas recessive mutations affecting the function of SCFTIR1 decrease auxin response. Here we show that SCFTIR1 is required for AUX/IAA degradation. We demonstrate that SCFTIR1 interacts with AXR2/IAA7 and AXR3/IAA17, and that domain II of these proteins is necessary and sufficient for this interaction. Further, auxin stimulates binding of SCFTIR1 to the AUX/IAA proteins, and their degradation. Because domain II is conserved in nearly all AUX/IAA proteins in Arabidopsis, we propose that auxin promotes the degradation of this large family of transcriptional regulators, leading to diverse downstream effects.

Item Type:	Article
Copyright, Publisher and Additional Information:	© 2001 Macmillan Magazines Ltd
Keywords:	SCF UBIQUITIN-LIGASE, ARABIDOPSIS-THALIANA, RESPONSE ELEMENTS, GENE, YEAST, TRANSCRIPTION, PATHWAY, FAMILY, NEDD8, RUB1
Academic Units:	The University of York > Biology (York)
Depositing User:	Repository Officer
Date Deposited:	14 Oct 2004
Last Modified:	19 Feb 2013 12:04
Published Version:	http://dx.doi.org/10.1038/35104500
Status:	Published
Refereed:	Yes
Related URLs:	http://www.nature.com/nature
URI:	http://eprints.whiterose.ac.uk/id/eprint/142

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